Composite

Part:BBa_K1918106:Design

Designed by: Linghan Wang   Group: iGEM16_Tsinghua-A   (2016-10-14)
Revision as of 12:36, 14 October 2016 by SheepW (Talk | contribs) (→‎Source)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


CMV-Kozak-EBFP2N(1-154):IntN


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1499
    Illegal SpeI site found at 83
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 755
    Illegal SpeI site found at 83
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 755
    Illegal BamHI site found at 738
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1499
    Illegal SpeI site found at 83
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1499
    Illegal SpeI site found at 83
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 716


Design Notes

AIM: EBFP2N(1-154):IntN could be expressed in cell all time; ELEMENTS: EBFP2N(1-154):IntN, CMV

Source

PCR from the existing plasmid in xie lab

References