Composite

Part:BBa_K1918101

Designed by: Linghan Wang   Group: iGEM16_Tsinghua-A   (2016-10-14)
Revision as of 12:14, 14 October 2016 by SheepW (Talk | contribs)


TRE-Kozak-EBFP2N(1-154)

This part is consist of EBFP2N(1-154) and the TRE promoter. EBFP2N(1-154) is a peptied made up with the 1st(N-terminus) to 154th amino acids in fluorescent protein EBFP2. TRE is the Dox induced promoter. Driven by TRE promoter, the expression of EBFP2C(155-238) could be induced by Dox.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 859
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 379
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 379
    Illegal BamHI site found at 362
    Illegal XhoI site found at 19
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 859
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 859
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None