Part:BBa_K2148010
mRasberry C.reinhardtii-CO far-red FP
This is a coding DNA sequence (CDS) for a far-red fluorescent protein, codon-optimised for Chlamydomonas reinhardtii chloroplast. It is coded as a CDS/CTAG Phytobrick.
Comparison of the colors of fluorescent proteins[1].
Usage and Biology
This is a level-0 Phytobrick conforming to the Phytobrick standard. It is coded as a CDS/CTAG Phytobrick and can be used with other level-0 Phytobricks to create a transcriptional unit that expresses a far-red fluorescent protein for Chlamydomonas reinhardtii chloroplast.
mRaspberry has excitation and emission maxima at 598 and 625 nm, respectively, and so should not overlap with chlorophyll's spectra. It has not been previously tested in the chloroplast of Chlamydomonas reinhardtii , but has the potential to be a novel reporter for it. The part has been codon-optimised, so its expression should be enhanced.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 231
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 231
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 231
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 231
- 1000COMPATIBLE WITH RFC[1000]
References
1. Zamai M. et al. (2008). Biomarkers and luminescent probes in quantitative biology. Biological and Medical Physics, Biomedical Engineering: Biophotonics (Springer), p. 182.
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