Coding

Part:BBa_K2040122

Designed by: Chilam Poon   Group: iGEM16_NYMU-Taipei   (2016-10-14)
Revision as of 11:41, 14 October 2016 by Chilam Poon (Talk | contribs)


mRFP1 + TtrpC

A SV40 nuclear localization signal was fused to the phototoxic protein KillerRed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1351
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1065


[edit]
Categories
//biosafety
//biosafety/kill_switch
//cds/reporter/rfp
//function/celldeath
//function/reporter/fluorescence
Parameters
colorRed
controlBBa_E1010 as a negative control
emission611 nm
excitation545-585 nm
functionPhototoxicity
originEngineered from anm2CP
outputSuperoxide radical anion
targetNucleus