Coding
Part:BBa_K2040122
Designed by: Chilam Poon Group: iGEM16_NYMU-Taipei (2016-10-14)
Revision as of 11:41, 14 October 2016 by Chilam Poon (Talk | contribs)
mRFP1 + TtrpC
A SV40 nuclear localization signal was fused to the phototoxic protein KillerRed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 715
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1351
Illegal AgeI site found at 555
Illegal AgeI site found at 667 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1065
[edit]
Categories
Parameters
//biosafety
//biosafety/kill_switch
//cds/reporter/rfp
//function/celldeath
//function/reporter/fluorescence
//biosafety/kill_switch
//cds/reporter/rfp
//function/celldeath
//function/reporter/fluorescence
color | Red |
control | BBa_E1010 as a negative control |
emission | 611 nm |
excitation | 545-585 nm |
function | Phototoxicity |
origin | Engineered from anm2CP |
output | Superoxide radical anion |
target | Nucleus |