Coding

Part:BBa_K1981001

Designed by: Yulei Dang, Xinhao Song   Group: iGEM16_NKU_China   (2016-10-14)
Revision as of 11:30, 14 October 2016 by Songxinhao (Talk | contribs)


lsrA

Autoinducer 2 import ATP-binding protein LsrA. Part of the ABC transporter complex LsrABCD involved in autoinducer 2 (AI-2) import. Regulation of uptake and processing of the quorum-sensing AI-2. Responsible for energy coupling to the transport system (Probable). This protein is essential for aerobic growth. In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

Construct AI-2 “consumer” by overexpressing the lsrA gene Bacillus amyloliquefaciens LL3.

This part is originated from Bacillus thuringiensis serovar thuringiensis str. IS5056.

Characterization

Electrophoresis

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Categories
Parameters
None