Regulatory
pGLN1

Part:BBa_K2030004:Design

Designed by: John Hellgren   Group: iGEM16_Chalmers_Gothenburg   (2016-10-14)
Revision as of 11:11, 14 October 2016 by Registry (Talk | contribs)

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pGLN1 S. cerevisiae promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 175
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 175
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 175
    Illegal BamHI site found at 735
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 175
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 175
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The complete sequence of Saccharomyces cerevisiae S288c chromosome XIV (Genbank accession number BK006947) was used to design primers for amplification of this promoter.


Source

Genomic DNA from Saccharomyces cerevisiae CEN.PK113-5D.

References