Regulatory

Part:BBa_K1970001:Design

Designed by: Eivind Bøe Drejer   Group: iGEM16_NTNU_Trondheim   (2016-10-14)
Revision as of 10:11, 14 October 2016 by Registry (Talk | contribs)

(diff) ↠Older revision | Latest revision (diff) | Newer revision → (diff)


XOR gate like repressor of CI repressor expression


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1374
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 337
    Illegal NheI site found at 1589
    Illegal SpeI site found at 1374
    Illegal NotI site found at 1381
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 615
    Illegal XhoI site found at 1867
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1374
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1374
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designing the deoxyribozyme ribonucleases so that they bound more tightly to each other than to CI repressor mRNA was challenging. Additionally, choosing which promoters to place in front of either gene required some thought on desired expression levels and controlability of the system.


Source

De novo DNA.

References