Device

Part:BBa_K1919301

Designed by: Shaobo Yang   Group: iGEM16_SCU-China   (2016-10-14)
Revision as of 09:59, 14 October 2016 by Registry (Talk | contribs)

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livF

LivF encodes a subunit of leucine ABC tansporter. This device constructed in our team contains a promoter J23100, a RBS, livF coding region and a terminator. The device can be transferred into E.coli and overexpress livF directly. The expression of the livF RNA in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livJ has been overexpressed in E.coli BL21 and E.coli DH5a.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None