Composite
Part:BBa_K1918104:Experience
Designed by: Linghan Wang Group: iGEM16_Tsinghua-A (2016-10-14)
Revision as of 07:51, 14 October 2016 by SheepW (Talk | contribs) (→Applications of BBa_K1918104: work fine)
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Applications of BBa_K1918104
1. Use PCR to get the DNA fragment of TRE and IntC:EBFP2C(155-238) 2. Ligate the fragment with vector 3. Heat shock transformation 4. Select colonies 5. Purification of plasmid DNA 7. Sequencing 8. Transfer into 293T cell 9. Use Dox to induce the expression of IntC:EBFP2C(155-238) 10. FACS
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