Part:BBa_K2113004

AMP without glycine

It consists of cationic anti-microbial peptides (AMP) with consecutive sequences of arginine and tryptophan (WRWRWR).This can be used as an antimicrobial agent against a wide range of gram positive and gram negative bacteria.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Mobyle@RPBS 3D Structure Prediction

(The following information has been contributed by SVCE_Chennai iGEM-2016)

3D Structure

ProtParam Results

(The following information has been contributed by SVCE_Chennai iGEM-2016)

Number of amino acids: 7 Molecular weight: 1176.41 Theoretical pI: 12.30 Total number of negatively charged residues (Asp + Glu): 0 Total number of positively charged residues (Arg + Lys): 3

Atomic composition:

Carbon (C) 56
Hydrogen (H) 77
Nitrogen (N) 19
Oxygen (O) 8
Sulfur (S) 1

Formula: C₅₆H₇₇N₁₉O₈S₁
Total number of atoms: 161

Extinction coefficients:

Extinction coefficients are in units of M^-1 cm^-1, at 280 nm measured in water.

Ext. coefficient: 16500
Abs 0.1% (=1 g/l): 14.026

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Instability index:

The instability index (II) is computed to be 172.23
This classifies the protein as unstable.

Aliphatic index: 0.00

Grand average of hydropathicity (GRAVY): -2.043