Composite

Part:BBa_K1918106

Designed by: Linghan Wang   Group: iGEM16_Tsinghua-A   (2016-10-14)
Revision as of 06:47, 14 October 2016 by Registry (Talk | contribs)

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CMV-Kozak-EBFP2N(1-154):IntN

This part is consist of the N-terminal coding region(1st-154th amino acid) of the fluorescent protein EBFP2 with intN and the CMV promoter. Thus, the expression of EBFP2N(1-154)-intN would be always.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1499
    Illegal SpeI site found at 83
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 755
    Illegal SpeI site found at 83
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 755
    Illegal BamHI site found at 738
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1499
    Illegal SpeI site found at 83
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1499
    Illegal SpeI site found at 83
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 716


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Categories
Parameters
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