Coding

Part:BBa_K2139002

Designed by: UBC iGEM 2016   Group: iGEM16_British_Columbia   (2016-10-13)
Revision as of 06:25, 14 October 2016 by Dchristy (Talk | contribs)


Coding Sequence of Gluc1C

BBa_K2139000 encompass the coding region for an 1,4-beta-glucosidase gene known as Gluc1C. Gluc1C is a monomeric enzyme capable of catalyzing the conversion of cellobiose into monomeric glucose at the non reducing end of the sugar polymer. It can be used in conjunction with an endoglucanase for the direct conversion of cellulose into monomeric glucose. The genbank ascension number for this protein is JQ713769.1 (DNA), AFQ36783.1 (amino acid), and a PDB code of 2O9R_A. This construct was synthesized for use in Caulobacter cresentus and is codon optimized to contain a high GC content.

Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 264
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1138


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Categories
Parameters
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