Reporter
GG96
Part:BBa_K2145001
Designed by: Melody Wu, Laura Xinyu Chen, Katie Maruna Group: iGEM16_Alverno_CA (2016-10-14)
GFP/RFP reporter (Reverse/Forward) with random 500bp spacer
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) along with a "spacer" part and pSB1C3 backbone assembled to make the final part. It can be used to express both GFP and RFP proteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2270
Illegal AgeI site found at 2382 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |