Coding

Part:BBa_K1972007

Designed by: Shuli Liang   Group: iGEM16_SCUT-China_A   (2016-10-13)
Revision as of 04:39, 14 October 2016 by Registry (Talk | contribs)

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dszBCAD with T7 promoter

We constructed dszBCAD genes with T7 promoter and choose E.coli BL21 as our host cell. When IPTG is added to our system, DszABCD enzymes will be successful expressed and these four enzymes convert dibenzothiophene (DBT) undergoes through three successive oxidation steps and one a hydrolytic step to 2-hydroxybiphenyl (2HBP) by the 4s pathway.

Each single enzyme gene was synthesized by Generay and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1267
    Illegal NheI site found at 4441
    Illegal NheI site found at 4489
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 489
    Illegal BamHI site found at 2071
    Illegal XhoI site found at 481
    Illegal XhoI site found at 3226
    Illegal XhoI site found at 4067
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 196
    Illegal NgoMIV site found at 2515
    Illegal NgoMIV site found at 2730
    Illegal NgoMIV site found at 3034
    Illegal NgoMIV site found at 3154
    Illegal AgeI site found at 2689
    Illegal AgeI site found at 4255
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2221
    Illegal SapI.rc site found at 3538


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