Coding

Part:BBa_K1972006

Designed by: Shuli Liang   Group: iGEM16_SCUT-China_A   (2016-10-13)
Revision as of 04:00, 14 October 2016 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


dszC (a monooxygenase gene ), E.coli-optimized

DszA, DszB, DszC and dszD are required by the 4S pathway, in which dibenzothiophene (DBT) undergoes three successive oxidation steps and one a hydrolytic step leading to the formation of 2-hydroxybiphenyl (2HBP). And three successive oxidation steps require reducing equivalents (FMNH2) supplied by a flavinreductase (DszD) while working.

This single flavinreductase gene was synthesized whith E.coli codon optimized by Generay and the sequence was found in NCBI (GenBank Accession number L37363.1)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 168
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 397
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None