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Part:BBa_I715002:Design

Designed by: Will DeLoache   Group: iGEM07_Davidson_Missouri_W   (2007-07-02)
Revision as of 18:46, 2 July 2007 by Wideloache (Talk | contribs) (→‎Design Notes)


pLac-lox-RBS-Tet (in pSB3K3)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 412
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 558
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 584
    Illegal NgoMIV site found at 952
    Illegal NgoMIV site found at 1112
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in plasmid pSB1A2. We also plan to move the part into the insulated plasmid pSB1A7 to eliminate the effects of read-through transcription.

Source

None

References