Composite

Part:BBa_K1997023

Designed by: Chushu Zhu   Group: iGEM16_NUDT_CHINA   (2016-10-13)
Revision as of 01:54, 14 October 2016 by Zhuchushu13 (Talk | contribs)


P+R->sLuc-N->FRB->RBS->FKBP->sLuc-C->TER

This part is an integrated tool for protein-protein interaction research using split-LUC system as reporter. the "FRB-RBS-FKBP" subpart can be easily replaced using Golden Gate technique with BsaI Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2158
    Illegal BsaI.rc site found at 1513


[edit]
Categories
//awards/part_collection/2016
Parameters
None