Composite
Part:BBa_K1997022
Designed by: Chushu Zhu Group: iGEM16_NUDT_CHINA (2016-10-13)
P+R->sLuc-N->Zif-N->RBS->Zif-C->sLuc-C->TER
This part is an integrated tool for protein-protein interaction research using split-LUC system as reporter. the "Zif-N->RBS->Zif-C" subpart can be easily replaced using Golden Gate technique with BsaI
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1609
Illegal AgeI site found at 1693
Illegal AgeI site found at 1813 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1816
Illegal BsaI.rc site found at 1513
[edit]
Categories
Parameters
//awards/part_collection/2016
None |