Composite

Part:BBa_K1997013

Designed by: Xinyuan Qiu   Group: iGEM16_NUDT_CHINA   (2016-10-13)
Revision as of 00:26, 14 October 2016 by Zhuchushu13 (Talk | contribs)


P+R->sHRP-N->Zif-N->RBS->Zif-C->sHRP-C->TER

This part is an integrated tool for protein-protein interaction research using split-HRP system as reporter. the "Zif-N->RBS->Zif-C" subpart can be easily replaced using Golden Gate technique with BsaI

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 382
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 624
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1003
    Illegal AgeI site found at 1087
    Illegal AgeI site found at 1207
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1210
    Illegal BsaI.rc site found at 907
    Illegal SapI.rc site found at 1324


[edit]
Categories
//awards/part_collection/2016
Parameters
None