Composite

Part:BBa_K1997015:Design

Designed by: Chushu Zhu   Group: iGEM16_NUDT_CHINA   (2016-10-13)
Revision as of 00:00, 14 October 2016 by Zhuchushu13 (Talk | contribs) (→‎Design Notes)

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P+R->sGFP-N->Zif268->sGFP-C->TER


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 832
    Illegal AgeI site found at 916
    Illegal AgeI site found at 1000
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1003
    Illegal BsaI.rc site found at 736
    Illegal BsaI.rc site found at 1217


Design Notes

We added BsaI sites at the flank of "Zif268" sequence so that this sequence can be easily replaced.

Source

This part is synthesized by company.

References