Coding

Part:BBa_K2020050

Designed by: Andrea Hoeltken, Carolina Bonerath, Vroni Czotscher   Group: iGEM16_Aachen   (2016-10-02)
Revision as of 23:06, 13 October 2016 by Andrea hoeltken (Talk | contribs)


wild type Mj Y-Synthetase for use in E.coli

This is the wild type tyrosyl synthetase derived from Methanococcus janaschii to be used as a orthogonal synthetase in E.coli. This part can be used together with the cognate tRNA BBa_K2020042 to incorporate Tyrosin in response to an amber stop codon.


Usage and Biology

Control for incorporation of ncAA

Assembly in a synthetase plasmid for incorporation of ncAA

pACYC derived plasmid with Mj tyrosyl synthetase and cognate tRNA


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 84
    Illegal SapI.rc site found at 877


[edit]
Categories
Parameters
None