Composite

Part:BBa_K2039000

Designed by: Maxence Pfeiffer   Group: iGEM16_Paris_Saclay   (2016-10-12)
Revision as of 20:37, 13 October 2016 by SylvieL (Talk | contribs)


Part expressing the dimerization protein FKBP and GFP10 (subunit of tripartite GFP)

This part is the complementary part of Bba_K2039001.

The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the medium. It is particularly useful for protein interaction studies. The FRB sequence has been modified in order to dimerize with a non toxic rapamycin analog (rapalog). The mutations introduced are : T2098L, K2095P, W2101F. It has also been codon optimized with Jcat plateforme to be expressed in bacteria.

FKBP protein is linked to GFP 10.

The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 546
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 157
    Illegal AgeI site found at 331
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None