RNA

Part:BBa_K1941004

Designed by: Dimitri Coukos   Group: iGEM16_EPFL   (2016-10-13)
Revision as of 20:05, 13 October 2016 by Registry (Talk | contribs)

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scCyc1_2PP7

Scaffold RNA that targets the region c6 of CYC1 promoter and recruits two times the protein repressor module PCP-Mxi via two effector protein recruitments site PP7. PP7 is a well-characterized viral RNA sequence which is recognized by PCP protein. This RNA is composed of the dCas9-binding sgRNA that interacts with CYC1, and two times the PCP-binding RNA hairpin, PP7.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 231
    Illegal NgoMIV site found at 260
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None