Regulatory
Part:BBa_K2148017
Designed by: Chern Juin Ong Group: iGEM16_Cambridge-JIC (2016-10-13)
C.reinhardtii chloroplast rbcL 3UTR w/ Homologies
This part contains the 3'UTR for the rbcL gene found in Chlamydomonas reinhardtii chloroplast genome and has the homology regions right from the trnE2-psbH integenic region with psaA promoter. This is coded as a level-0 3UTR/TERM Phytobrick, which together with other level-0 Phytobrick can form a desired transcriptional unit for transformation, which would also be available for Homologous Recombination.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 515
Illegal NheI site found at 1944
Illegal NheI site found at 2265 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 77
Illegal BsaI.rc site found at 2670
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Categories
Parameters
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