DNA

Part:BBa_K1951008:Design

Designed by: claire raynaud   Group: iGEM16_Aix-Marseille   (2016-10-13)
Revision as of 19:35, 13 October 2016 by Registry (Talk | contribs)

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FliC E.coli producer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1285
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 362
    Illegal AgeI site found at 770
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

On the BbaK1951004, the restriction site Bbs1 is mutated (substitution) to allow the control for a future Bbs1 site insertion.


Source

We made this part from 2 others :

- BbaK1951004

- Bba880005

References