Composite

Part:BBa_K2120306

Designed by: HongYu Yang   Group: iGEM16_BIT-China   (2016-10-13)
Revision as of 18:28, 13 October 2016 by YangHongYu (Talk | contribs)


B0015+CI+B0034+pBAD-AraC+B0015 pBAD is a promoter induced by arabinose and arac is the repressor. We use it to test the expression of reporter gene in our validation circuit .And CI is an inhibitor which is built to repress the expression of PR promoter. We use it to control the “on” or ”off” state of toxin gene. In order to avoid leaking out, We reserve【BBa_K2120305 】.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1001
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1166
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1183


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Categories
Parameters
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