Device

Part:BBa_K2120301:Design

Designed by: HongYu Yang   Group: iGEM16_BIT-China   (2016-10-13)
Revision as of 16:25, 13 October 2016 by YangHongYu (Talk | contribs) (→‎Design Notes)

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pR+B0032+RFP+B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 631
    Illegal AgeI site found at 743
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The PR promoter has two DNA binding sites for lambada CI repressor BBa_C0051.and RFP is the reporter gene to test the inhibit efficiency of PR promoter.In our project,the reporter gene is regarded as the killer gene.We use it in our validation circuit.

Source

References