Composite

Part:BBa_K1997016:Design

Designed by: Chushu Zhu   Group: iGEM16_NUDT_CHINA   (2016-10-13)
Revision as of 14:16, 13 October 2016 by Zhuchushu13 (Talk | contribs) (→‎Design Notes)


P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 832
    Illegal AgeI site found at 916
    Illegal AgeI site found at 1036
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1039
    Illegal BsaI.rc site found at 736
    Illegal BsaI.rc site found at 1253


Design Notes

We added BsaI sites at the flank of "Zif-N->RBS->Zif-C" sequence so that this sequence can be easily replaced.

Source

We obtained it through the contribution of our basic subparts.

References