Composite

Part:BBa_K1997016

Designed by: Chushu Zhu   Group: iGEM16_NUDT_CHINA   (2016-10-13)
Revision as of 14:15, 13 October 2016 by Zhuchushu13 (Talk | contribs)


P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER

This part is a negative control of GFP reporter system with the split Zif268.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 832
    Illegal AgeI site found at 916
    Illegal AgeI site found at 1036
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1039
    Illegal BsaI.rc site found at 736
    Illegal BsaI.rc site found at 1253


[edit]
Categories
//awards/part_collection/2016
Parameters
None