Regulatory

Part:BBa_K1935008:Design

Designed by: Jean DESCARPENTRIE   Group: iGEM16_Bordeaux   (2016-10-13)
Revision as of 13:18, 13 October 2016 by Jdesca911e (Talk | contribs) (→‎References)

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pMYO-2 : Specific Pharynx muscle promoter of C.elegans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 580
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 490


Design Notes

A mutagenesis was done to abolish one illegal EcoRI restriction sites (317-323).



Source

PROMOTEROME : http://worfdb.dfci.harvard.edu/promoteromedb/searchPromoterome.pl?by=name&sid=myo-2

References

Wormbase : http://www.wormbase.org/species/c_elegans/gene/WBGene00003514#01-9g-3