Composite

Part:BBa_K1937003:Experience

Designed by: Oumnia KARIM   Group: iGEM16_Toulouse_France   (2016-10-13)
Revision as of 12:40, 13 October 2016 by Oumniak (Talk | contribs) (→‎Applications of BBa_K1937003)

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Applications of BBa_K1937003

The part was sub-cloned in thepSBBsOK-mini plasmid (BBa_K1937001) for validation (resulting in part BBa_K1937004).In presence of N-acetyl glucosamine in the media, the clones are expected to be red, and white without this substrate Nag.

BBa K1937003-results.png

We indeed observed some red stains on the colonies only with the pNagA promoter growing on NAG (as well as with the pNagP promoter, see part BBa_K1937006), but the expression is late during the colonies development and does not seem to be specific (same result with the control plate on glucose)

User Reviews

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