RNA

Part:BBa_K1946002

Designed by: Musa Efe Işılak   Group: iGEM16_Bilkent-UNAMBG   (2016-10-13)
Revision as of 11:29, 13 October 2016 by Registry (Talk | contribs)

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sgRNA targeting LacI

This part has a sgRNA targeting lacI. It also has 5' and 3' ribozyme which cleave the RNA molecule with self catalysis so that any sequence can be added to 5' or 3' to this part without preventing Cas9 binding and its activity.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 43
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 43
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 43
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 43
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 43
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/crispr/cas9
Parameters
targetLacI