Coding
Part:BBa_K1998001:Design
Designed by: Shauna Winchester Group: iGEM16_Macquarie_Australia (2016-10-13)
YCF54 - ChlM
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 912
Illegal BamHI site found at 316 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 478
Design Notes
Codon optimisation, order of parts in operon, illegal enzyme kinetics
Source
The genes were sourced from Chlamydomonas rienhardtii and synthesised.