Composite

Part:BBa_K1916102:Design

Designed by: Jonathan Chen   Group: iGEM16_UC_Davis   (2016-10-12)
Revision as of 04:07, 13 October 2016 by Registry (Talk | contribs)

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Ara-Inducible Cyan7427_1390g3 Expression System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 731
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1251
    Illegal AgeI site found at 1341
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.


Source

Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.

References