Device

Part:BBa_K1879001

Designed by: Madeleine Stone   Group: iGEM16_Virginia   (2016-10-12)
Revision as of 03:38, 13 October 2016 by Registry (Talk | contribs)

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promoter + CBZ cleavage enzyme + terminator

This part contains the CBZ-cleavage enzyme with a promoter (BBa_J23118) and RBS upstream of the coding sequence, and terminator (BBa_J61048) downstream of the sequence to make a fully functioning BioBrick. This BioBrick is used in our biocontainment system to cleave the CBZ protecting group from leucine prior to incorporation of leucine into proteins. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1402
    Illegal AgeI site found at 354
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 56
    Illegal BsaI.rc site found at 1377


[edit]
Categories
Parameters
None