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Part:BBa_K2150018

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)
Revision as of 01:30, 13 October 2016 by Registry (Talk | contribs)

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pT7+pTet+RBS+GFP+DT

Two promoters are used, a constitutive promoter pTet and a T7RNAP specific promoter pT7. By testing the fluorescence intensity, we found that in the absence of T7 promoter, this part exhibits higher efficiency than single pTet.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 752


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