Composite

Part:BBa_K2019002

Designed by: John Lazar   Group: iGEM16_Northwestern   (2016-10-12)
Revision as of 01:28, 13 October 2016 by T lazar (Talk | contribs)


mRFP + sgRNA targeting mRFP Coding Region (saCas9)

Part BBa_K2019002 consists of constitutively expressed mRFP gene (BBa_J04550) along with a constitutively expressed sgRNA that targets the mRFP gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1099
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1076
    Illegal SpeI site found at 1099
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1099
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1099
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None