Composite
Part:BBa_K1900002:Design
Designed by: Elise Sloey Group: iGEM16_WLC-Milwaukee (2016-10-12)
pBAD+strong RBS+E. coli tolC signal sequence+E. coli tolC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 265
Illegal NheI site found at 1615
Illegal NheI site found at 1636 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1555
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 874
Illegal NgoMIV site found at 1144 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1110
Design Notes
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Source
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