Composite
Part:BBa_K1900001:Design
Designed by: Elise Sloey Group: iGEM16_WLC-Milwaukee (2016-10-12)
pBAD+strong RBS+E. coli tolC signal sequence+S. marcescens tolC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1642
Illegal NheI site found at 1663 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1582
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 337
Illegal NgoMIV site found at 1126
Illegal NgoMIV site found at 1413
Illegal AgeI site found at 1311 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
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Source
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