Composite

Part:BBa_K2019002:Design

Designed by: John Lazar   Group: iGEM16_Northwestern   (2016-10-12)
Revision as of 00:24, 13 October 2016 by Registry (Talk | contribs)

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mRFP + sgRNA targeting mRFP Coding Region (saCas9)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1099
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1076
    Illegal SpeI site found at 1099
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1099
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1099
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Considering that this construct would have to be cotransformed into a cell line with saCas9 expressed in the BBa_R0040-PSB1C3 backbone, all tests of this construct were in the PSB1T3 backbone.


Source

The guide insert was designed using Benchling's gRNA design tool.

References