Coding

Part:BBa_K1998007

Designed by: Shauna Winchester   Group: iGEM16_Macquarie_Australia   (2016-10-12)
Revision as of 23:47, 12 October 2016 by Registry (Talk | contribs)

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psbMZHWK-OPQR

This part is composed of two operons in the photosystem II pathway. The psbM, psbZ, psbH, psbW and psbK genes. The psbM protein subunit is positioned at the monomer-monomer interface. The psbZ protein controls the interaction of Photosystem II cores with the light-harvesting antenna. The psbH protein is required for stability and assembly of the photosystem II complex. The psbW protein stabilizes dimeric photosytem II. The psbK protein is also required for stability and assembly of Photosystem II. The second operon is composed of the psbO, psbP, psbQ and psbR genes. The psbO gene that comprises this part ecodes a manganese stabilizing protein. The psbP gene that comprises this part transcribes a protein that optimizes the availability of Ca2+ and Cl- cofactors in the Oxygen Evolving Complex in PSII to maintain the active Manganese cluster. The psbQ protein is an oxygen enhancer protein. The psbR protein is an important link in the Photosystem II core complex for stable assembly of the Oxygen Evolving Complex.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3063
    Illegal BglII site found at 3392
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 570
    Illegal NgoMIV site found at 1819
    Illegal NgoMIV site found at 2465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3456


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Categories
Parameters
None