Coding

Part:BBa_K2019000

Designed by: John Lazar   Group: iGEM16_Northwestern   (2016-07-15)
Revision as of 23:21, 12 October 2016 by T lazar (Talk | contribs)


Histidine Tagged WT Staphylococcus Aureus Cas9 Protein

Part BBa_K2019000 consists of a histidine tagged wild-type staphylococcus aureus CRISPR associated protein 9 (saCas9) codon optimized for escherichia coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 627
    Illegal BglII site found at 780
    Illegal BglII site found at 926
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 200
    Illegal AgeI site found at 2756
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None