Coding
MymTsfGFP

Part:BBa_K1980003

Designed by: Sam Garforth   Group: iGEM16_Oxford   (2016-10-11)
Revision as of 20:43, 12 October 2016 by Sgarforth (Talk | contribs)


MymT sfGFP

MymT is a small prokaryotic copper metallothein discovered in Mycobacterium tuberculosis. It can bind up to 7 copper ions but has a preference for 4-6. This version has a C terminal sfGFP with a C terminal hexahistidine tag for purification. The MymT and sfGFP are separated by a small flexible linker. This part has been codon optimised for E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 595
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/metal
//cds/reporter/gfp
Parameters
None