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Part:BBa_K2074008:Design

Designed by: Hao Feng   Group: iGEM16_FAFU-CHINA   (2016-10-09)
Revision as of 19:11, 12 October 2016 by Heao (Talk | contribs) (→‎Design Notes)


Cry4Aa(Codon optimization)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 986
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1378
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 31
    Illegal BsaI.rc site found at 184
    Illegal BsaI.rc site found at 2071
    Illegal BsaI.rc site found at 2611
    Illegal BsaI.rc site found at 2920
    Illegal BsaI.rc site found at 3487
    Illegal SapI.rc site found at 1188
    Illegal SapI.rc site found at 1230


Design Notes

UUU  5.0(  2110)  UCU  4.7(  1992)  UAU  2.6(  1085)  UGU  1.4(   601)
UUC 27.1( 11411)  UCC 16.1(  6782)  UAC 22.8(  9579)  UGC 13.1(  5498)
UUA  0.6(   247)  UCA  3.2(  1348)  UAA  1.0(   441)  UGA  0.5(   227)
UUG  4.0(  1673)  UCG 16.1(  6763)  UAG  0.4(   183)  UGG 13.2(  5559)

CUU  4.4(  1869)  CCU  8.1(  3416)  CAU  2.2(   919)  CGU  4.9(  2071)
CUC 13.0(  5480)  CCC 29.5( 12409)  CAC 17.2(  7252)  CGC 34.9( 14676)
CUA  2.6(  1086)  CCA  5.1(  2124)  CAA  4.2(  1780)  CGA  2.0(   841)
CUG 65.2( 27420)  CCG 20.7(  8684)  CAG 36.3( 15283)  CGG 11.2(  4711)

AUU  8.0(  3360)  ACU  5.2(  2171)  AAU  2.8(  1157)  AGU  2.6(  1089)
AUC 26.6( 11200)  ACC 27.7( 11663)  AAC 28.5( 11977)  AGC 22.8(  9590)
AUA  1.1(   443)  ACA  4.1(  1713)  AAA  2.4(  1028)  AGA  0.7(   287)
AUG 25.7( 10796)  ACG 15.9(  6684)  AAG 43.3( 18212)  AGG  2.7(  1150)

GUU  5.1(  2158)  GCU 16.7(  7030)  GAU  6.7(  2805)  GGU  9.5(  3984)
GUC 15.4(  6496)  GCC 54.6( 22960)  GAC 41.7( 17519)  GGC 62.0( 26064)
GUA  2.0(   857)  GCA 10.6(  4467)  GAA  2.8(  1172)  GGA  5.0(  2084)
GUG 46.5( 19558)  GCG 44.4( 18688)  GAG 53.5( 22486)  GGG  9.7(  4087)

Source

1

References