Device

Part:BBa_K1886006:Design

Designed by: Lejian Jiang   Group: iGEM16_ZJU-China   (2016-10-12)
Revision as of 17:07, 12 October 2016 by Registry (Talk | contribs)

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broken Ptet-cph8


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2332
    Illegal XhoI site found at 439
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There was a SpeI site in the sequence encoding protein cph8, so we designed a silent mutation.



Source

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References