Coding

Part:BBa_K1989028

Designed by: Yiming Dong   Group: iGEM16_Peking   (2016-10-12)
Revision as of 15:06, 12 October 2016 by Registry (Talk | contribs)

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Triple spytag with mSA, His-tag , FlAsH-tag and signal peptide PhoA

The fused protein PhoA-Histag-3A-mSA-FlAsHtag possess both isopeptide bond forming function, secretion and collectable. Thus, using PhoA-Histag-3A- mSA -FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 547
    Illegal AgeI site found at 607
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None