Coding
Part:BBa_K1989028
Designed by: Yiming Dong Group: iGEM16_Peking (2016-10-12)
Triple spytag with mSA, His-tag , FlAsH-tag and signal peptide PhoA
The fused protein PhoA-Histag-3A-mSA-FlAsHtag possess both isopeptide bond forming function, secretion and collectable. Thus, using PhoA-Histag-3A- mSA -FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 547
Illegal AgeI site found at 607 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |