Reporter

Part:BBa_K2150016

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)
Revision as of 14:54, 12 October 2016 by Freedom.H@UCAS (Talk | contribs)


pTet TetX-GFP(fusion protein)

T7 promoter and RBS and GFP, with a double terminator. Though it has been reported that T7 promoter doesn't work with E.coli RNA polymerase but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 642
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1939


[edit]
Categories
Parameters
None