Part:BBa_K1555000:Experience

This sensor can be activated in low copper concentration and it's truely useful.

2h 0mg/L copper ion 2h 1mg/L copper ion

Applications of BBa_K1555000

This year we add the RiboJ(a section of functional RNA used to buffer synthetic circuits from genetic context) between the copA pomoter and the RBS(B0030) as an improvement.Also we used GFP as the reporter. For more details please visit the compsite part BBa_K2088015 copA+riboJ+B0030+GFP+B0015. Also BBa_K2088014 J23100+riboJ+M6+GFP+B0015, M6 belongs to riboswitch, is an another improvement. For more details please check BBa_K2088015 and BBa_K2088014.

2016 NAU-CHINA

User Reviews

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••••• HSiTAIWAN

Detecting Copper in Chinese medicine Objective      Measure the E. coli fluorescence intensity in different copper ion concentration with 3μg/ml Chinese medicine. Methods      Copper ion solution with different copper ion concentration (0, 3, 9, 27ppm) is added into E. coli DH5α with Plasmids encoding GFP under copper promoter(part: BBa_K1555000).      Fluorescence was measured at: Excitation peak: 470nm Emission peak: 508nm every 30 minutes. Results      This part is still specific to copper in Chinese medicine, so we can apply it in Chinese medicine examination. Discussion      Since E. coli expresses stronger fluorescence in higher copper concentration, this is a relevant promoter to detect the existence and measure the amount of copper. Review This part works great! HSiTAIWAN iGEM team 2016 used this part to detect copper ions in Chinese Medicine. The results were as expected. The reporter performed well without being interfered by complex substances. This part performed in an expected pattern and is an useful reporter.

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