Composite
Part:BBa_K1991010
Designed by: Chen, Pei-En Group: iGEM16_Mingdao (2016-10-12)
Pcons-RBS-LO-GFP-His
A study showed that Lpp-OmpA (LO) hybrid can direct the heterologous protein GFP to the external surface of E. coli (Enzyme Microb Technol. 2001)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 1220 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 497
Illegal XhoI site found at 1229 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 452
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1143
[edit]
Categories
Parameters
None |