Composite

Part:BBa_K1991010

Designed by: Chen, Pei-En   Group: iGEM16_Mingdao   (2016-10-12)
Revision as of 09:30, 12 October 2016 by Registry (Talk | contribs)

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Pcons-RBS-LO-GFP-His

A study showed that Lpp-OmpA (LO) hybrid can direct the heterologous protein GFP to the external surface of E. coli (Enzyme Microb Technol. 2001)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 1220
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 497
    Illegal XhoI site found at 1229
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 452
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1143


[edit]
Categories
Parameters
None