Generator

Part:BBa_K2014010

Designed by: Daria Niewiadomska   Group: iGEM16_UAM_Poznan   (2016-10-12)
Revision as of 09:25, 12 October 2016 by Registry (Talk | contribs)

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pxylS-E1_5'UTR->sfGFP_W

We were using xylS promoter to compare the influence of different ORFs structures coding for the same protein sfGFP. The xylS promoter is a modified version of xylR-xylF-xylA promoter from E.coli K-12 genome.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 203
    Illegal SpeI site found at 338
    Illegal SpeI site found at 515
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 203
    Illegal SpeI site found at 338
    Illegal SpeI site found at 515
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 203
    Illegal SpeI site found at 338
    Illegal SpeI site found at 515
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 203
    Illegal SpeI site found at 338
    Illegal SpeI site found at 515
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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